We developed a method, 3-prime mRNA extension sequencing (PME-seq), for bulk RNA-seq profiling. We use a “fragment RNA first” approach followed by barcoded oligo(dT) priming for cDNA synthesis and sample pooling prior to sequencing library construction. While PME-seq is applicable to any RNA sample, including in low amounts (<1 ng per sample), we also created procedures to collect, store and lyse a dozen mouse organ types using conditions compatible with downstream high-throughput and low-cost RNA extraction and sequencing with PME-seq.

Original publications: Kadoki et al., Cell, 2017 & Pandey et al., Nature Protocols, 2020